Post by Lyme Challenged on Nov 21, 2018 6:48:06 GMT -5
I asked dr. G about the different forms of Bb and what they actually mean, here is his response, thanks.
Thomas Grier Well even I am confused because of terms that we often use interchangeably that are vague and undefined. But Dr. Lida Mattman PhD wrote about early work using different sized pore-filters to separate particles. Granules are the smallest and when separated with filters, Bjorson could culture them into classical spiral forms. Blebs are pinched off from a classical spiral form and Dr. Claude Garon NIH RML concluded that they usually;;y contain the full compliment of Bb DNA and in the same ratio.
Some feel Blebs are a defense mechanism to survive , others think Blebs confuse the Mammalian Immune system, Regardless of their purpose Dr. Judith Miklossy MD PhD routinely saw them in human brains and when the Bb was cultured from brains they tended to produce blebs and biofilms more easily than Bb cultured from skin??? The their are Lister Forms or True L-Forms. Here the classical spiral form sheds its peptidoglycan cell wall (Its a very thin layer compared to something like mycobacteria) when it shed its cell wall the spiral shape cannot be maintained, so the least energy form it takes is a sphere. These can be quite large and in many species of Borrelia like B. vincentii (1958), and after B. burgdorferi - classical spiral forms can sometimes be seen inside a L-Form.
Is this a form of replication? We never seem to see plain old binary fission in Borrelia in cultures.so how do they replicate? One thing we know from Rocky Mountain Lab - Claude Garon is that at least 300 copies of its entire genome is embedded on the inner membrane. So Blebs pinching off also take with it DNA. How the DNA is structured is not understood because we identify circular and linear plasmids and a big linear chromosome, but unlike other bacteria these chromosomes are not floating in the cytoplasm waiting for the prokaryote equivalent of mitosis.
Thomas Grier Blebs observed from Borrelia isolated from Human Alzheimer's brain and then cultured - by Judith Miklossy (Her work is extraordinary and the next step was to add these Bb isolates from human brains into RAT Brain cultures, and the result was in 8 weeks we see eight major markers for Alzheimer's. This is the closest in-vitro model of Alzheimer's ever created.
Thomas Grier Well even I am confused because of terms that we often use interchangeably that are vague and undefined. But Dr. Lida Mattman PhD wrote about early work using different sized pore-filters to separate particles. Granules are the smallest and when separated with filters, Bjorson could culture them into classical spiral forms. Blebs are pinched off from a classical spiral form and Dr. Claude Garon NIH RML concluded that they usually;;y contain the full compliment of Bb DNA and in the same ratio.
Some feel Blebs are a defense mechanism to survive , others think Blebs confuse the Mammalian Immune system, Regardless of their purpose Dr. Judith Miklossy MD PhD routinely saw them in human brains and when the Bb was cultured from brains they tended to produce blebs and biofilms more easily than Bb cultured from skin??? The their are Lister Forms or True L-Forms. Here the classical spiral form sheds its peptidoglycan cell wall (Its a very thin layer compared to something like mycobacteria) when it shed its cell wall the spiral shape cannot be maintained, so the least energy form it takes is a sphere. These can be quite large and in many species of Borrelia like B. vincentii (1958), and after B. burgdorferi - classical spiral forms can sometimes be seen inside a L-Form.
Is this a form of replication? We never seem to see plain old binary fission in Borrelia in cultures.so how do they replicate? One thing we know from Rocky Mountain Lab - Claude Garon is that at least 300 copies of its entire genome is embedded on the inner membrane. So Blebs pinching off also take with it DNA. How the DNA is structured is not understood because we identify circular and linear plasmids and a big linear chromosome, but unlike other bacteria these chromosomes are not floating in the cytoplasm waiting for the prokaryote equivalent of mitosis.
Thomas Grier Blebs observed from Borrelia isolated from Human Alzheimer's brain and then cultured - by Judith Miklossy (Her work is extraordinary and the next step was to add these Bb isolates from human brains into RAT Brain cultures, and the result was in 8 weeks we see eight major markers for Alzheimer's. This is the closest in-vitro model of Alzheimer's ever created.